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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_CS417149
Database: nrnl1 Go to original record
Description: Sequence 109 from Patent WO2006094976. Sequence 109 from Patent EP2045327. Sequence 109 from patent US 8088971. Sequence 109 from Patent EP2166099. Sequence 109 from Patent EP2166100. Sequence 109 from Patent EP2166101. Sequence 109 from Patent EP2166102. Sequence 109 from Patent EP2169058.
Taxon: unidentified
Original size: 6794 bp
Patents: WO2006094976 (lens, espacenet) EP2045327 (lens, espacenet) US8088971 (lens, espacenet) EP2166099 (lens, espacenet) EP2166100 (lens, espacenet) EP2166101 (lens, espacenet) EP2166102 (lens, espacenet) EP2169058 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 962 1030 - 69 bp
Amplicon: 69 bp 
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa

View other 15381 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.