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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM716131
Database: nrnl1 Go to original record
Description: Sequence 159 from Patent WO2008100545. Sequence 482 from Patent WO2008124048. Sequence 30 from Patent WO2008128240. Sequence 159 from patent US 8013215. Sequence 30 from patent US 8119860. Sequence 59 from patent US 8188338. Sequence 59 from Patent WO2008124806.
Taxon: unidentified
Original size: 7872 bp
Patents: WO2008100545 (lens, espacenet) WO2008124048 (lens, espacenet) WO2008128240 (lens, espacenet) US8013215 (lens, espacenet) US8119860 (lens, espacenet) US8188338 (lens, espacenet) WO2008124806 (lens, espacenet)

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Method: QL-ELE-00-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim CaMV P-35S) (Waiblinger et al., 2008).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 3904 3985 - 82 bp
Amplicon: 82 bp 
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

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Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.