JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_GM716131
Database:	nrnl1	Go to original record
Description:	Sequence 159 from Patent WO2008100545. Sequence 482 from Patent WO2008124048. Sequence 30 from Patent WO2008128240. Sequence 159 from patent US 8013215. Sequence 30 from patent US 8119860. Sequence 59 from patent US 8188338. Sequence 59 from Patent WO2008124806.
Taxon:	unidentified
Original size:	7872 bp
Patents:	WO2008100545 (lens, espacenet) WO2008124048 (lens, espacenet) WO2008128240 (lens, espacenet) US8013215 (lens, espacenet) US8119860 (lens, espacenet) US8188338 (lens, espacenet) WO2008124806 (lens, espacenet)

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Method:	QL-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3790	3912	-	123 bp

Amplicon:

123 bp

1     ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.84e-09
primerB	100.0	25 bp	0	0	123	99	1.30e-11

Match details