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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM953047
Database: nrnl1 Go to original record
Description: Sequence 1 from Patent WO2008142034. Sequence 8433 from Patent WO2008142034. Sequence 2 from Patent WO2009027539. Sequence 1 from Patent WO2009037279. Sequence 14389 from Patent WO2009037279. Sequence 1 from Patent WO2009037329. Sequence 6064 from Patent WO2009037329. Sequence 1 from Patent WO2009077611. Sequence 2902 from Patent WO2009077611. Sequence 11 from Patent WO2009106596. Sequence 41 from Patent WO2010020654. Sequence 41 from Patent WO2010034672. Sequence 4 from Patent WO2010037714. Sequence 41 from Patent WO2010046221.
Taxon: unidentified
Original size: 9045 bp
Patents: WO2008142034 (lens, espacenet) WO2008142034 (lens, espacenet) WO2009027539 (lens, espacenet) WO2009037279 (lens, espacenet) WO2009037279 (lens, espacenet) WO2009037329 (lens, espacenet) WO2009037329 (lens, espacenet) WO2009077611 (lens, espacenet) WO2009077611 (lens, espacenet) WO2009106596 (lens, espacenet) WO2010020654 (lens, espacenet) WO2010034672 (lens, espacenet) WO2010037714 (lens, espacenet) WO2010046221 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 2326 2394 + 69 bp
Amplicon: 69 bp 
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa

View other 15381 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.