Match details

Record ID: NRN_GM961480
Database: nrnl1 Go to original record
Description: Sequence 8434 from Patent WO2008142034. Sequence 14393 from Patent WO2009037279. Sequence 6065 from Patent WO2009037329. Sequence 2903 from Patent WO2009077611. Sequence 12 from Patent WO2009106596. Sequence 42 from Patent WO2010020654. Sequence 42 from Patent WO2010034672. Sequence 5 from Patent WO2010037714. Sequence 42 from Patent WO2010046221.
Taxon: unidentified
Original size: 9466 bp
Patents: WO2008142034 (lens, espacenet) WO2009037279 (lens, espacenet) WO2009037329 (lens, espacenet) WO2009077611 (lens, espacenet) WO2009106596 (lens, espacenet) WO2010020654 (lens, espacenet) WO2010034672 (lens, espacenet) WO2010037714 (lens, espacenet) WO2010046221 (lens, espacenet)

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Method: QL-ELE-00-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2707 2790 + 84 bp

Amplicon: 84 bp
1     catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51    cgcaattata catttaatac gcgatagaaa acaa 

View other 30799 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 25 bp 0 0 1 25 9.20e-12
primerB 100.0 25 bp 0 0 84 60 9.20e-12
probe 100.0 28 bp 0 0 28 55 2.28e-13


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.