Match details

Record ID: NRN_GM979720
Database: nrnl1 Go to original record
Description: Sequence 124 from Patent WO2008147935. Sequence 124 from patent US 8143473. Sequence 215 from patent US 8143476. Sequence 343 from patent US 8153859. Sequence 343 from Patent WO2009143401. Sequence 215 from Patent WO2009143398.
Taxon: unidentified
Original size: 11615 bp
Patents: WO2008147935 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 1290 1358 - 69 bp

Amplicon: 69 bp
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
51    agcgcgcaaa ctaggataa 

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Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.84e-13
primerB 96.0 25 bp 1 0 69 45 9.65e-11


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.