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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GN081606
Database: nrnl1 Go to original record
Description: Sequence 4 from Patent WO2009029739. Sequence 34 from patent US 7812223. Sequence 34 from patent US 8158859. Sequence 4 from Patent WO2009058947. Sequence 4 from Patent WO2009061776. Sequence 4 from Patent WO2009067569. Sequence 34 from Patent WO2009073605. Sequence 4 from Patent WO2009079529. Sequence 43 from Patent WO2009086229. Sequence 4 from Patent WO2009126359. Sequence 4 from Patent WO2009132057. Sequence 4 from Patent WO2010006010. Sequence 4 from Patent WO2010011907. Sequence 4 from Patent WO2010019872. Sequence 4 from Patent WO2010053621. Sequence 4 from Patent WO2010077890. Sequence 4 from Patent WO2010104848. Sequence 4 from Patent WO2010144276. Sequence 4 from Patent WO2011046772. Sequence 71 from Patent WO2011053897. Sequence 4 from Patent EP2511374. Sequence 4 from Patent WO2012087903.
Taxon: unidentified
Original size: 16843 bp
Patents: WO2009029739 (lens, espacenet) US7812223 (lens, espacenet) US8158859 (lens, espacenet) WO2009058947 (lens, espacenet) WO2009061776 (lens, espacenet) WO2009067569 (lens, espacenet) WO2009073605 (lens, espacenet) WO2009079529 (lens, espacenet) WO2009086229 (lens, espacenet) WO2009126359 (lens, espacenet) WO2009132057 (lens, espacenet) WO2010006010 (lens, espacenet) WO2010011907 (lens, espacenet) WO2010019872 (lens, espacenet) WO2010053621 (lens, espacenet) WO2010077890 (lens, espacenet) WO2010104848 (lens, espacenet) WO2010144276 (lens, espacenet) WO2011046772 (lens, espacenet) WO2011053897 (lens, espacenet) EP2511374 (lens, espacenet) WO2012087903 (lens, espacenet)

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Method: QL-ELE-00-017 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Barbau-Piednoir et al., 2014).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 11472 11546 - 75 bp
Amplicon: 75 bp 
1     aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac
51    aatcccacta tccttcgcaa gaccc

View other 1050 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 1.23e-09
primerB 100.0 20 bp 0 0 75 56 3.68e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.