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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM979658
Database: nrnl1 Go to original record
Description: Sequence 62 from Patent WO2008147935. Sequence 21 from patent US 8101819. Sequence 62 from patent US 8143473. Sequence 62 from patent US 8143476. Sequence 62 from patent US 8153859. Sequence 62 from Patent WO2009143401. Sequence 62 from Patent WO2009143398. Sequence 21 from Patent WO2009143397.
Taxon: unidentified
Original size: 10866 bp
Patents: WO2008147935 (lens, espacenet) US8101819 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet) WO2009143397 (lens, espacenet)

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Method: QT-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Feinberg et al., 2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2667 2745 - 79 bp
Amplicon: 79 bp 
1     cgtcttcaaa gcaagtggat tgatgtgaca tctccactga cgtaagggat
51    gacgcacaat cccactatcc ttcgcaaga

View other 3624 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 22 bp 0 0 1 22 3.41e-10
primerB 100.0 22 bp 0 0 79 58 3.41e-10
probe 100.0 26 bp 0 0 31 56 2.52e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.