European Commission logo
Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_GM979701
Database: nrnl1 Go to original record
Description: Sequence 105 from Patent WO2008147935. Sequence 105 from patent US 8143473. Sequence 196 from patent US 8143476. Sequence 324 from patent US 8153859. Sequence 324 from Patent WO2009143401. Sequence 196 from Patent WO2009143398.
Taxon: unidentified
Original size: 8358 bp
Patents: WO2008147935 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet)

View other 11 matches for this record

Method: QT-ELE-00-004 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter
Target:CaMV P-35S

View other 15625 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 2450 2531 + 82 bp
Amplicon: 82 bp 
1     gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51    cgtggaaaaa gaagacgttc caaccacgtc tt

View other 26489 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 18 bp 0 0 1 18 4.54e-08
primerB 100.0 22 bp 0 0 82 61 3.55e-10
probe 100.0 22 bp 0 0 21 42 3.55e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.