JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_GM979644
Database:	nrnl1	Go to original record
Description:	Sequence 48 from Patent WO2008147935. Sequence 48 from patent US 8143473. Sequence 48 from patent US 8143476. Sequence 48 from patent US 8153859. Sequence 48 from Patent WO2009143401. Sequence 48 from Patent WO2009143398. Sequence 129 from Patent WO2011062748.
Taxon:	unidentified
Original size:	8720 bp
Patents:	WO2008147935 (lens, espacenet) US8143473 (lens, espacenet) US8143476 (lens, espacenet) US8153859 (lens, espacenet) WO2009143401 (lens, espacenet) WO2009143398 (lens, espacenet) WO2011062748 (lens, espacenet)

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Method:	QL-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3696	3818	+	123 bp

Amplicon:

123 bp

1     ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.84e-09
primerB	100.0	25 bp	0	0	123	99	1.30e-11

Match details