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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: NRN_FB724507
Database: nrnl1 Go to original record
Description: Sequence 915 from Patent WO2007149406. JP 2010530745-A/6: MICROORGANISMS FOR IMAGING AND/OR TREATMENT OF TUMORS. Sequence 6 from Patent WO2008156655. Sequence 40 from patent US 8052968. Sequence 40 from patent US 8066984. Sequence 394 from Patent WO2009054996. Sequence 40 from Patent WO2008100292. Sequence 40 from Patent WO2009139921. Sequence 122 from Patent WO2009139921. Sequence 116 from Patent WO2010054010. JP 2012508017-A/114: Anti-DLL4 Antibodies and Uses Thereof.
Taxon: unidentified
Original size: 3519 bp
Patents: WO2007149406 (lens, espacenet) WO2007149406 (lens, espacenet) JP2010530745 (lens, espacenet) WO2008156655 (lens, espacenet) US8052968 (lens, espacenet) US8066984 (lens, espacenet) WO2009054996 (lens, espacenet) WO2008100292 (lens, espacenet) WO2009139921 (lens, espacenet) WO2009139921 (lens, espacenet) WO2010054010 (lens, espacenet) JP2012508017 (lens, espacenet)

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Method: QL-ELE-00-003 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998)
Target:nptII

View other 58813 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1536 1708 + 173 bp
Amplicon: 173 bp 
1     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
51    ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
101   gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
151   cggtcttgtc gatcaggatg atc

View other 57974 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 16 bp 0 0 1 16 1.08e-06
primerB 100.0 16 bp 0 0 173 158 1.08e-06

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.