JRC GMO-Amplicons | EURL GMFF

Record ID:	NRN_FB706319
Database:	nrnl1	Go to original record
Description:	Sequence 26 from Patent WO2007127381. MUTANT DELTA-8 DESATURASE GENES ENGINEERED BY TARGETED MUTAGENSIS AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS. Sequence 68 from Patent WO2008063340. Sequence 185 from Patent WO2008073271. Sequence 185 from Patent WO2008073275. Sequence 185 from patent US 7709239. Sequence 68 from patent US 7863502. Sequence 26 from patent US 7943823. Sequence 185 from patent US 8017838. Sequence 185 from patent US 8026089. Sequence 26 from patent US 8058517. Sequence 26 from Patent EP2405012.
Taxon:	unidentified
Original size:	7887 bp
Patents:	WO2007127381 (lens, espacenet) WO2007127381 (lens, espacenet) JP2010512146 (lens, espacenet) WO2008063340 (lens, espacenet) WO2008073271 (lens, espacenet) WO2008073275 (lens, espacenet) US7709239 (lens, espacenet) US7863502 (lens, espacenet) US7943823 (lens, espacenet) US8017838 (lens, espacenet) US8026089 (lens, espacenet) US8058517 (lens, espacenet) EP2405012 (lens, espacenet)

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Method:	QL-ELE-00-004	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (Lipp et al.,2001).
Target:	CaMV P-35S

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	3178	3300	+	123 bp

Amplicon:

123 bp

1     ccacgtcttc aaagcaagtg gattgatgtg acatctccac tgacgtaagg
51    gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
101   aagttcattt catttggaga gga

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Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	21 bp	0	0	1	21	1.84e-09
primerB	100.0	25 bp	0	0	123	99	1.30e-11

Match details