Match details
| Record ID: | JN949143.1 | |
|---|---|---|
| Database: | emblreltgn | Go to original record |
| Description: | Mus musculus targeted deletion, lacZ-tagged mutant allele Smad1:tm1(KOMP)Ucd; transgenic. | |
| Taxon: | Mus musculus | |
| Original size: | 37222 bp | |
View other 3 matches for this record
| Method: | QL-ELE-00-003 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of Neomycin phosphotransferase II gene (BVL L 00.00-31, 1998) | |
| Target: | nptII | |
View other 58813 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 21066 | 21238 | + | 173 bp |
| Amplicon: | 173 bp |
|---|
1 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 51 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 101 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 151 cggtcttgtc gatcaggatg atc
View other 57974 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 16 bp | 0 | 0 | 1 | 16 | 1.08e-06 |
| primerB | 100.0 | 16 bp | 0 | 0 | 173 | 158 | 1.08e-06 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.