Match details
| Record ID: |
KY464890.1 |
| Database: |
emblreltgn |
Go to original record |
| Description: |
Nicotiana benthamiana transgenic NptII (aph(3')-II (or nptII)) and modified green fluorescent protein mGFP5-ER genes, complete cds; and transposon Tn5393 TnpA (tnpA) and TnpR (tnpR) genes, complete cds. |
| Taxon: |
Nicotiana benthamiana |
| Original size: |
9484 bp |
View other 45 matches for this record
View other 1572 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 0 |
0 |
1781 |
1943 |
+ |
163 bp |
|
1 ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
51 tctgcaccgg atctggatcg tttcgcatga ttgaacaaga tggattgcac
101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
151 acaacagaca atc
View other 978 matches with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
23 bp |
0 |
0 |
1 |
23 |
2.01e-10 |
| primerB |
100.0 |
22 bp |
0 |
0 |
163 |
142 |
6.81e-10 |
| probe |
100.0 |
25 bp |
0 |
0 |
140 |
116 |
1.74e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
