Match details
Record ID: KF036176.1
Database:
emblreltgn
Go to original record
Description:
Oryza sativa Indica Group transgenic cultivar Bengal phosphinothricin-acetyl-transferase (bar) gene, complete cds.
Taxon:
Oryza sativa Indica Group
Original size:
2494 bp
View other 23 matches for this record
Method: QL-CON-00-015
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the CaMV35S promoter and the bar gene (verified by the EURL GMFF in the context of Commission Decision 2006/578/EC).
Target: CaMV P-35S-bar
View other 358 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
1096
1198
+
103 bp
1 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
51 gaggacaggg taccggatcc aacaatgagc ccagaacgac gcccggccga
101 cat
View other 5 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
1.53e-09
primerB
100.0
22 bp
0
0
103
82
4.52e-10
probe
100.0
25 bp
0
0
22
46
1.14e-11
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
