Match details
| Record ID: | JN950419.1 | |
|---|---|---|
| Database: | emblreltgn | Go to original record |
| Description: | Mus musculus targeted KO-first, conditional ready, lacZ-tagged mutant allele Hadhb:tm1a(KOMP)Wtsi; transgenic. | |
| Taxon: | Mus musculus | |
| Original size: | 37985 bp | |
View other 3 matches for this record
| Method: | QL-ELE-00-002 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005). | |
| Target: | nptII | |
View other 59426 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 21079 | 21293 | + | 215 bp |
| Amplicon: | 215 bp |
|---|
1 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 51 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 101 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 151 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 201 gccaggctca aggcg
View other 57490 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 21 bp | 0 | 0 | 1 | 21 | 3.06e-09 |
| primerB | 100.0 | 21 bp | 0 | 0 | 215 | 195 | 3.06e-09 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.