Match details

Record ID: GX747623.1
Database: emblrelpat Go to original record
Description: Sequence 15802 from patent US 7834146.
Taxon: unidentified
Original size: 1529 bp
Patents: US7834146 (lens, espacenet)

Method: QT-TAX-ZM-004 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of maize aldolase 1 gene.
Target:maize aldolase 1

View other 211 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 335 403 - 69 bp

Amplicon: 69 bp
1     agggaggacg cctccctcct tgaggacatc gacaaaaggc ttgccatcct
51    tggtcttctg gtagagggt 

View other 29 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 17 bp 0 0 1 17 1.38e-07
primerB 95.5 22 bp 1 0 69 48 1.37e-08
probe 96.2 26 bp 1 0 21 46 1.01e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.