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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: EA757026.1
Database: emblrelpat Go to original record
Description: Sequence 21 from patent US 7371940.
Taxon: unidentified
Original size: 9356 bp
Patents: US7371940 (lens, espacenet)

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Method: QT-EVE-GH-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event COT102 (EURL GMFF, 2020).
Target:SYN-IR102-7

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 8805 8905 + 101 bp
Amplicon: 101 bp 
1     tctccgctca tgatcagatt gtcgtttccc gccttcagtt taaactatca
51    gtgtttaata aatatgggca atctttccct acaccgactg tactgttact
101   g

View other 5 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.30e-10
primerB 100.0 25 bp 0 0 101 77 1.12e-11
probe 100.0 33 bp 0 0 27 59 5.34e-16

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.