Match details
Record ID: |
EA419704.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
Sequence 24 from patent US 7323556. |
Taxon: |
unidentified |
Original size: |
7390 bp |
Patents: |
US7323556 (lens, espacenet)
|
View other 12 matches for this record
Method: |
QT-CON-00-005 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator. |
Target: | pat-CaMV T-35S |
View other 222 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
6885 |
7033 |
+ |
149 bp |
|
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgcccgc
51 tgaaatcacc agtctctctc tacaaatcta tctctctcta taataatgtg
101 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctca
View other 150 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
21 bp |
0 |
0 |
1 |
21 |
2.09e-09 |
primerB |
100.0 |
24 bp |
0 |
0 |
149 |
126 |
5.39e-11 |
probe |
100.0 |
22 bp |
0 |
0 |
36 |
57 |
6.20e-10 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.