Match details

Record ID: EA419703.1
Database: emblrelpat Go to original record
Description: Sequence 23 from patent US 7323556.
Taxon: unidentified
Original size: 11922 bp
Patents: US7323556 (lens, espacenet)

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Method: QT-CON-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target:pat-CaMV T-35S

View other 222 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 9456 9604 + 149 bp

Amplicon: 149 bp
1     gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgcccgc
51    tgaaatcacc agtctctctc tacaaatcta tctctctcta taataatgtg
101   tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctca 

View other 150 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.09e-09
primerB 100.0 24 bp 0 0 149 126 5.39e-11
probe 100.0 22 bp 0 0 36 57 6.20e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.