Match details
Record ID: |
DI091422.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
Novel insecticidal toxins derived from Bacillus thuringiensis insecticidalcrystal proteins. |
Taxon: |
unidentified |
Original size: |
11127 bp |
View other 12 matches for this record
Method: |
QL-ELE-00-013 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative duplex PCR method for detection of Cauliflower Mosaic Virus 35S promoter and nopaline synthase terminator (partim T-nos) (Waiblinger et al., 2008). |
Target: | T-nos |
View other 15584 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
6404 |
6487 |
+ |
84 bp |
|
1 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
51 cgcaattata catttaatac gcgatagaaa acaa
View other 30799 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
25 bp |
0 |
0 |
1 |
25 |
9.20e-12 |
primerB |
100.0 |
25 bp |
0 |
0 |
84 |
60 |
9.20e-12 |
probe |
100.0 |
28 bp |
0 |
0 |
28 |
55 |
2.28e-13 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.