Match details
Record ID: |
DI055447.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
Novel insecticidal toxins derived from Bacillus thuringiensis insecticidalcrystal proteins. |
Taxon: |
unidentified |
Original size: |
14603 bp |
View other 7 matches for this record
Method: |
QT-CON-00-007 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Quantitative PCR method for detection of the junction between a synthetic cry1A(b) gene and the Phospho-enol-pyruvate Carboxylase intron N.9. |
Target: | cryIAb-PEPC |
View other 15 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
1916 |
2032 |
+ |
117 bp |
|
1 tgttcaccag cagcaaccag atcggcctga agaccgacgt gaccgactac
51 cacatcgacc aggtgagcaa cctggtggac tgcttaagct agagatctgt
101 tctgcacaaa gtggagt
View other 3 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
20 bp |
0 |
0 |
1 |
20 |
5.89e-09 |
primerB |
100.0 |
25 bp |
0 |
0 |
117 |
93 |
1.23e-11 |
probe |
100.0 |
25 bp |
0 |
0 |
34 |
58 |
1.23e-11 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.