Match details
| Record ID: |
DD278615.1 |
| Database: |
emblrelpat |
Go to original record |
| Description: |
The reagent for measuring quantity of nucleic acid,and measuring method using this. |
| Taxon: |
unidentified |
| Original size: |
128 bp |
View other 4 matches for this record
| Method: |
QT-TAX-GM-001 |
Go to GMOMETHODS record |
| Description: | Quantitative PCR method for detection of soybean lectin gene |
| Target: | soybean lectin |
View other 39 matches for this method
| Predicted amplification details: |
| Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
| 1 |
0 |
15 |
95 |
+ |
81 bp |
|
1 tccaccccca tccacatttg ggacaaagaa accggtagcg ttgccagctt
51 cgccgcttgc ttcaacttca ccttctatgc c
View other 1 match with an identical amplicon
| Primers and probe alignment: |
| Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
| primerA |
100.0 |
19 bp |
0 |
0 |
1 |
19 |
1.34e-08 |
| primerB |
100.0 |
22 bp |
0 |
0 |
81 |
60 |
3.91e-10 |
| probe |
100.0 |
23 bp |
0 |
0 |
30 |
52 |
1.03e-10 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.
