Match details
Record ID:
BD105559.1
Database:
emblrelpat
Go to original record
Description:
Method of constructing transgenic plant accompanied by biosynthesis of modified 5-aminolevulinic acid and method of identifying 5-aminolevulinic acid synthesis effector.
Taxon:
unidentified
Original size:
4186 bp
Patents:
JP2001526041 (lens , espacenet )
View other 12 matches for this record
Method:
QL-CON-00-005
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of the junction between the pat gene and the CaMV35S terminator (ISO/FDIS 21569:2005).
Target: pat-CaMV T-35S
View other 212 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
345
535
-
191 bp
1 atggtggatg gcatgatgtt ggtttttggc aaagggattt tgagttgcca
51 gctcctccaa ggccagttag gccagttacc cagatctgac gctgaaatca
101 ccagtctctc tctacaaatc tatctctctc tataataatg tgtgagtagt
151 tcccagataa gggaattagg gttcttatag ggtttcgctc a
View other 3 matches with an identical amplicon
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.71e-09
primerB
100.0
23 bp
0
0
191
169
2.37e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.