Match details

Record ID: A05521.1
Database: emblrelpat Go to original record
Description: pRT100 DNA sequence
Taxon: synthetic construct
Original size: 711 bp
Patents: WO8809374 (lens, espacenet)

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Method: QL-ELE-00-005 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (ISO/FDIS 21569:2005).
Target:CaMV P-35S

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 194 389 + 196 bp

Amplicon: 196 bp
1     gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagaa
51    tgcctctacc gacagtggtc ccaaagatgg acccccaccc acgaggaaca
101   tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
151   tgtgatatct ccactgacgt aagggatgac gcacaatccc actatc 

View other 185 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 94.7 19 bp 1 0 1 19 1.45e-06
primerB 100.0 20 bp 0 0 196 177 9.33e-09


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.