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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MY364836.1
Database: emblrelpat Go to original record
Description: Sequence 1 from patent US 11085050.
Taxon: unidentified
Original size: 9328 bp
Patents: US11085050 (lens, espacenet)

View other 1 match for this record

Method: QT-EVE-GH-013 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of cotton event GHB811 (EURL GMFF, 2020).
Target:BCS-GH811-4
Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1162 1305 + 144 bp
Amplicon: 144 bp 
1     cgaatagttc catcaatttt atcatttatg tatttggaag ccttgaaaca
51    gaacaacagt actgggcccc ctcgagggcg atcgctacgt acctgcaggc
101   ccgggttaat taaggcccga tcaaatctga gggacgttaa agcg
Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 30 bp 0 0 1 30 3.21e-14
primerB 100.0 22 bp 0 0 144 123 5.98e-10
probe 100.0 18 bp 0 0 38 55 8.20e-08

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.