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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: HW295710.1
Database: emblrelpat Go to original record
Description: JP 2013522300-A/20: FMD VIRUS RECOMBINANT VACCINES AND USES THEREOF.
Taxon: synthetic construct
Original size: 16821 bp
Patents: JP2013522300 (lens, espacenet)

View other 9 matches for this record

Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 15244 15408 - 165 bp
Amplicon: 165 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccagatct
51    cgactctagt cgagggccca tgggagcttg gattgaacaa gatggattgc
101   acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
151   gcacaacaga caatc

View other 133 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.04e-10
primerB 100.0 22 bp 0 0 165 144 6.90e-10
probe 100.0 25 bp 0 0 142 118 1.76e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.