European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record HZ345421.1 details
Record ID: HZ345421.1
Database: emblrelpat Go to original record
Description: JP 2015519051-A/27: CORN EVENT MON 87411.
Taxon: synthetic construct
Original size: 12322 bp
Patents: JP2015519051 (lens, espacenet)

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Method QL-ELE-00-001 details
Method: QL-ELE-00-001 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998).
Target: CaMV P-35S

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 2677 2871 - 195 bp

Amplicon size
Amplicon: 195 bp
1     GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCCAT CGTTGAAGAT
51    GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT
101   CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT
151   GTGATATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATC 

View other 7603 matches with an identical amplicon


Primers and probe alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 2871 2853 4.42e-07
primerA 100.0 19 bp 0 0 3132 3114 4.42e-07
primerB 100.0 20 bp 0 0 2677 2696 1.22e-07


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.