European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record HZ163766.1 details
Record ID: HZ163766.1
Database: emblrelpat Go to original record
Description: JP 2015133970-A/42: COMPOSITIONS AND METHODS OF USE FOR MGD-CSF IN DISEASE TREATMENT.
Taxon: synthetic construct
Original size: 5472 bp
Patents: JP2015133970 (lens, espacenet)

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Method QL-ELE-00-002 details
Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target: nptII

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Predicted amplification details:

Predicted amplification details
Mismatches Gaps From To Strand Amplicon size
0 0 2776 2990 + 215 bp

Amplicon size
Amplicon: 215 bp
1     CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
51    CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
101   ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC
151   AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC
201   GCCAGGCTCA AGGCG 

View other 55585 matches with an identical amplicon


Primers alignment:

Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 2776 2796 1.48e-08
primerB 100.0 21 bp 0 0 2990 2970 1.48e-08


Related publications:

  • Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
  • Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.