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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MX531855.1
Database: emblrelpat Go to original record
Description: Sequence 29 from patent US 10973229.
Taxon: unidentified
Original size: 15294 bp
Patents: US10973229 (lens, espacenet)

View other 3 matches for this record

Method: QT-EVE-GM-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event DAS-81419-2 (EURL GMFF, 2015).
Target:DAS-81419-2

View other 35 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1356 1460 + 105 bp
Amplicon: 105 bp 
1     tctagctata tttagcactt gatattcatg aatcaaactt ctctatgaaa
51    taaccgcggt gcgcatcggt gcctgttgat cccgcgcaag ttgggatctt
101   gaagc

View other 35 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 29 bp 0 0 1 29 7.93e-14
primerB 100.0 21 bp 0 0 105 85 1.56e-09
probe 100.0 24 bp 0 0 80 57 3.98e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.