Match details
| Record ID: | A17370.1 | |
|---|---|---|
| Database: | emblrelpat | Go to original record |
| Description: | Artificial promoter-,polylinker- and terminatorsequence | |
| Taxon: | synthetic construct | |
| Original size: | 793 bp | |
| Patents: | EP0469273 (lens, espacenet) | |
View other 6 matches for this record
| Method: | QL-ELE-00-001 | Go to GMOMETHODS record |
|---|---|---|
| View in JRC GMO-Matrix | ||
| Description: | Qualitative PCR method for detection of Cauliflower Mosaic Virus 35S promoter (BVL L 00.00-31, 1998). | |
| Target: | CaMV P-35S | |
View other 10093 matches for this method
Predicted amplification details:
| Mismatches | Gaps | From | To | Strand | Amplicon size |
|---|---|---|---|---|---|
| 0 | 0 | 289 | 483 | + | 195 bp |
| Amplicon: | 195 bp |
|---|
1 GCTCCTACAA ATGCCATCAT TGCGATAAAG GAAAGGCCAT CGTTGAAGAT 51 GCCTCTGCCG ACAGTGGTCC CAAAGATGGA CCCCCACCCA CGAGGAGCAT 101 CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGCAA GTGGATTGAT 151 GTGATATCTC CACTGACGTA AGGGATGACG CACAATCCCA CTATC
View other 7603 matches with an identical amplicon
Primers alignment:
| Primer/Probe | % Identity | Length | Mismatches | Gaps | From | To | Score |
|---|---|---|---|---|---|---|---|
| primerA | 100.0 | 19 bp | 0 | 0 | 289 | 307 | 3.34e-08 |
| primerB | 100.0 | 20 bp | 0 | 0 | 483 | 464 | 9.09e-09 |
Related publications:
- Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
- Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.