Match details

Record ID: DL476432.1
Database: emblrelpat Go to original record
Description: CORN EVENT DAS-59122-7 AND METHODS FOR DETECTION THEREOF.
Taxon: synthetic construct
Original size: 555 bp
Patents: JP2008514233 (lens, espacenet)

Method: QT-EVE-ZM-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event 59122 (Mazzara et al., 2006).
Target:DAS-59122-7

View other 71 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 385 470 + 86 bp

Amplicon: 86 bp
1     gggataagca agtaaaagcg ctcaaacact gatagtttaa actgaaggcg
51    ggaaacgaca atctgatcat gagcggagaa ttaagg 

View other 71 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.10e-10
primerB 100.0 24 bp 0 0 86 63 3.22e-11
probe 100.0 26 bp 0 0 36 61 2.76e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.