Match details
Record ID:
DI435822.1
Database:
emblrelpat
Go to original record
Description:
KR 1020140135576-A/2: Standard Plasmid for Detection of Genetically Modified Organism, Qualitative detection method using the same and Detection Kit comprising the same.
Taxon:
synthetic construct
Original size:
1278 bp
Patents:
KR1020140135576 (lens , espacenet )
View other 10 matches for this record
Method:
QT-TAX-GM-020
Go to GMOMETHODS record
Description: Quantitative PCR method for detection of soybean lectin gene
Target: soybean lectin
View other 11 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
722
823
+
102 bp
1 ctttctcgca ccaattgaca ctaagccaca aacacatgca ggttatcttg
51 gtcttttcaa cgaaaacgag tctggtgatc aagtcgtcgc tgttgagttt
101 ga
View other 23 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
20 bp
0
0
1
20
5.10e-09
primerB
100.0
20 bp
0
0
102
83
5.10e-09
probe
100.0
26 bp
0
0
26
51
3.30e-12
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.