Match details
Record ID:
DI435822.1
Database:
emblrelpat
Go to original record
Description:
KR 1020140135576-A/2: Standard Plasmid for Detection of Genetically Modified Organism, Qualitative detection method using the same and Detection Kit comprising the same.
Taxon:
synthetic construct
Original size:
1278 bp
Patents:
KR1020140135576 (lens , espacenet )
View other 10 matches for this record
Method:
QT-TAX-GM-007
Go to GMOMETHODS record
Description: Quantitative PCR method for detection of soybean lectin gene
Target: soybean lectin
View other 38 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
597
714
+
118 bp
1 gccctctact ccacccccat ccacatttgg gacaaagaaa ccggtagcgt
51 tgccagcttc gccgcttcct tcaacttcac cttctatgcc cctgacacaa
101 aaaggcttgc agatgggc
View other 92 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
19 bp
0
0
1
19
1.99e-08
primerB
100.0
20 bp
0
0
118
99
5.94e-09
probe
100.0
26 bp
0
0
55
80
3.63e-12
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.