Match details
Record ID:
DI435809.1
Database:
emblrelpat
Go to original record
Description:
KR 1020140135575-A/1: Standard Plasmid for Detection of Genetically Modified Organism, Qualitative detection method using the same and Detection Kit comprising the same.
Taxon:
synthetic construct
Original size:
6922 bp
Patents:
KR1020140135575 (lens , espacenet )
View other 11 matches for this record
View other 15625 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
5936
6017
-
82 bp
1 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
51 cgtggaaaaa gaagacgttc caaccacgtc tt
View other 26489 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
18 bp
0
0
1
18
4.54e-08
primerB
100.0
22 bp
0
0
82
61
3.55e-10
probe
100.0
22 bp
0
0
21
42
3.55e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.