Match details
Record ID:
DI435809.1
Database:
emblrelpat
Go to original record
Description:
KR 1020140135575-A/1: Standard Plasmid for Detection of Genetically Modified Organism, Qualitative detection method using the same and Detection Kit comprising the same.
Taxon:
synthetic construct
Original size:
6922 bp
Patents:
KR1020140135575 (lens , espacenet )
View other 11 matches for this record
Method:
QT-CON-00-004
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the intron 1 from the maize hsp70 gene and a synthetic cry1A(b) gene.
Target: IVS-HSP-cryIAb
View other 13 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
4953
5065
-
113 bp
1 gatgccttct ccctagtgtt gaccagtgtt actcacatag tctttgctca
51 tttcattgta atgcagatac caagcggcca tggacaacaa cccaaacatc
101 aacgagtgca tcc
View other 13 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
22 bp
0
0
1
22
4.98e-10
primerB
100.0
21 bp
0
0
113
93
1.68e-09
probe
100.0
26 bp
0
0
65
90
3.47e-12
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.