Match details

Record ID: DI389542.1
Database: emblrelpat Go to original record
Description: KR 1020140007352-A/27: STACKED HERBICIDE TOLERANCE EVENT 8264.44.06.1, RELATED TRANSGENIC SOYBEAN LINES, AND DETECTION THEREOF.
Taxon: synthetic construct
Original size: 13659 bp
Patents: KR1020140007352 (lens, espacenet)

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Method: QT-EVE-GM-015 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event DAS-44406-6 (EURL GMFF, 2015).
Target:DAS-44406-6

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1435 1533 + 99 bp

Amplicon: 99 bp
1     ttattgttct tgttgtttcc tctttaggaa cttacatgta aacggtaagg
51    tcatcatgga ggtccgaata gtttgaaatt agaaagctcg caattgagg 

View other 13 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 2.56e-13
primerB 100.0 24 bp 0 0 99 76 3.74e-11
probe 100.0 29 bp 0 0 69 41 7.45e-14


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.