Match details
Record ID: |
DI232613.1 |
Database: |
emblrelpat |
Go to original record |
Description: |
KR 1020120003853-A/5: RICE TRANSGENIC EVENT 17053 AND METHODS OF USE THEREOF. |
Taxon: |
artificial sequences |
Original size: |
3142 bp |
Patents: |
KR1020120003853 (lens, espacenet)
|
View other 15 matches for this record
Method: |
QL-CON-00-008 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative PCR method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (Grohmann et al., 2009). |
Target: | CTP2-CP4 EPSPS |
View other 257 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
1 |
0 |
1422 |
1509 |
+ |
88 bp |
|
1 gggatgacgt taattggctc tgagcttcgt cctcttaagg tcatgtcttc
51 tgtttccacg gcgtgcatgc tacacggtgc aagcagcc
View other 20 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
3.82e-10 |
primerB |
94.7 |
19 bp |
1 |
0 |
88 |
70 |
6.82e-07 |
probe |
100.0 |
27 bp |
0 |
0 |
65 |
39 |
8.22e-13 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.