Match details

Record ID: OF002214.1
Database: emblrelpat Go to original record
Description: KR 1020200047746-A/88: METHODS AND COMPOSITIONS FOR INTEGRATION OF AN EXOGENOUS SEQUENCE WITHIN THE GENOME OF PLANTS.
Taxon: synthetic construct
Original size: 6661 bp
Patents: KR1020200047746 (lens, espacenet)

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Method: QL-ELE-00-021 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of phosphinothricin N-acetyltransferase (pat) gene (Barbau-Piednoir et al., 2014).
Target:pat

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
1 0 712 820 - 109 bp

Amplicon: 109 bp
1     ccgcggtttg tgatatcgtt aaccattaca ttgagacgtc tacagtgaac
51    tttaggacag agccacaaac accacaagag tggattgatg atctcgagag
101   gttgcaaga 

View other 74 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 5.47e-09
primerB 96.0 25 bp 1 0 109 85 5.64e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.