Match details

Record ID: MP977795.1
Database: emblrelpat Go to original record
Description: Sequence 46 from Patent EP3790593.
Taxon: synthetic construct
Original size: 33532 bp
Patents: EP3790593 (lens, espacenet)

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Method: QT-EVE-ZM-031 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event DP915635 (EURL GMFF, 2023).
Target:DP-915635-5

View other 5 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 25069 25168 + 100 bp

Amplicon: 100 bp
1     gcatctagga ccgactagct aactaactag taccgaggcc ggccccgcgg
51    gagctcggcg cgccatgagg agcaatcatt gttcaagaca tgatgcaaag  

View other 3 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 27 bp 0 0 1 27 9.41e-13
primerB 100.0 24 bp 0 0 100 77 3.78e-11
probe 100.0 15 bp 0 0 61 75 2.00e-06


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.