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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MP782880.1
Database: emblrelpat Go to original record
Description: Sequence 7 from Patent EP3717650.
Taxon: synthetic construct
Original size: 1350 bp
Patents: EP3717650 (lens, espacenet)
Method: QT-EVE-ZM-030 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of maize event MON 87429 (EURL GMFF, 2021).
Target:MON-87429-9

View other 5 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 958 1073 + 116 bp
Amplicon: 116 bp 
1     cgagacagac tcaatgtatc cgagatactc ttgtttggtt acacttgcaa
51    aaatagacca ctcttgtttg gtttcatgtc cgggaaatct acatggatca
101   gcaatgagta tgatgg

View other 5 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 30 bp 0 0 1 30 2.56e-14
primerB 100.0 27 bp 0 0 116 90 1.04e-12
probe 100.0 30 bp 0 0 85 56 2.56e-14
probe 100.0 14 bp 0 0 40 27 2.00e-05

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.