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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: MP412332.1
Database: emblrelpat Go to original record
Description: Sequence 71 from Patent WO2019224336.
Taxon: synthetic construct
Original size: 16875 bp
Patents: WO2019224336 (lens, espacenet)

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Method: QL-CON-00-014 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of the junction between the nos promoter and the neomycin phosphotransferase II gene
Target:P-nos-nptII

View other 1572 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 925 1093 - 169 bp
Amplicon: 169 bp 
1     ttcccctcgg tatccaatta gagtctcata ttcactctca atccatacaa
51    aaacacatcc aaaatcacag aaaatgggag cttggattga acaagatgga
101   ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
151   ctgggcacaa cagacaatc

View other 13 matches with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 2.09e-10
primerB 100.0 22 bp 0 0 169 148 7.07e-10
probe 100.0 25 bp 0 0 146 122 1.80e-11

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.