Match details

Record ID:	MP389678.1
Database:	emblrelpat	Go to original record
Description:	Sequence 30 from Patent WO2019209700.
Taxon:	synthetic construct
Original size:	79 bp
Patents:	WO2019209700 (lens, espacenet)

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Method:	QT-TAX-ZM-008	Go to GMOMETHODS record
Description:	Quantitative PCR method for detection of maize high-mobility-group gene
Target:	maize high-mobility-group

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Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	79	+	79 bp

Amplicon:

79 bp

1     ttggactaga aatctcgtgc tgattaattg ttttacgcgt gcgtttgtgt
51    ggattgtagg acaaggctcc ctatgtagc

View other 35 matches with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	23 bp	0	0	1	23	1.00e-10
primerB	100.0	22 bp	0	0	79	58	3.41e-10
probe	100.0	23 bp	0	0	56	34	1.00e-10

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.