Match details

Record ID:	MP385269.1
Database:	emblrelpat	Go to original record
Description:	Sequence 33 from Patent WO2019211296.
Taxon:	synthetic construct
Original size:	267 bp
Patents:	WO2019211296 (lens, espacenet)

Method:	QL-ELE-00-008	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of nopaline synthase promoter.
Target:	P-nos

View other 3921 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	1	130	223	+	94 bp

Amplicon:

94 bp

1     aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
51    cactatcagc tagcaaatat ttcttgtcaa aatgctccac tgac

View other 1 match with an identical amplicon

Primers and probe alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	26 bp	0	0	1	26	3.03e-12
primerB	95.8	24 bp	0	1	93	71	5.93e-09
probe	100.0	26 bp	0	0	28	53	3.03e-12

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.