Match details

Record ID: HV307961.1
Database: emblrelpat Go to original record
Description: JP 2010029196-A/6: Improvements Relating to the Specificity of Gene Expression.
Taxon: synthetic construct
Original size: 1559 bp
Patents: JP2010029196 (lens, espacenet)

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Method: QL-ELE-00-018 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of nopaline synthase terminator (Barbau-Piednoir et al., 2014).
Target:T-nos

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
2 1 1420 1489 + 70 bp

Amplicon: 70 bp
1     gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatta
51    tgcgcgcaaa ctaggataaa  

View other 11 matches with an identical amplicon


Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 28 bp 0 0 1 28 1.87e-13


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.