Match details

Record ID: MD245289.1
Database: emblrelpat Go to original record
Description: JP 2020108358-A/13: A method for detecting a target subject with high sensitivity.
Taxon: synthetic construct
Original size: 3931 bp
Patents: JP2020108358 (lens, espacenet)

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Method: QT-TAX-GM-001 Go to GMOMETHODS record
Description:Quantitative PCR method for detection of soybean lectin gene
Target:soybean lectin

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Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 58 138 - 81 bp

Amplicon: 81 bp
1     tccaccccca tccacatttg ggacaaagaa accggtagcg ttgccagctt
51    cgccgcttcc ttcaacttca ccttctatgc c 

View other 30 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 19 bp 0 0 1 19 1.34e-08
primerB 100.0 24 bp 0 0 81 58 3.03e-11
probe 100.0 23 bp 0 0 30 52 1.03e-10


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.