Match details

Record ID: MA129296.1
Database: emblrelpat Go to original record
Description: JP 2018500885-A/269: MATERIALS AND METHODS FOR PUFA PRODUCTION, AND PUFA-CONTAINING COMPOSITIONS.
Taxon: synthetic construct
Original size: 44910 bp
Patents: JP2018500885 (lens, espacenet)

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Method: QT-EVE-BN-012 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of oilseed rape event LBFLFK locus1 (EURL GMFF, 2022).
Target:BPS-BFLFK-2 locus1

View other 39 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 44275 44402 + 128 bp

Amplicon: 128 bp
1     accatcatac tcattgctga tccatgtaga tttcccggac atgaagccat
51    atatctgacc ctactccaca aatatatttt tatttataaa aaggtggcca
101   ttgtatacta tgtgtgcgta tacaggaa 

View other 10 matches with an identical amplicon


Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 23 bp 0 0 1 23 1.56e-10
primerB 100.0 25 bp 0 0 128 104 1.35e-11
probe 100.0 26 bp 0 0 24 49 3.95e-12


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.