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European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: LZ176620.1
Database: emblrelpat Go to original record
Description: JP 2017515509-A/1: Evolution Of High-Titer Virus-Like Vesicles For Vaccine Applications.
Taxon: synthetic construct
Original size: 13590 bp
Patents: JP2017515509 (lens, espacenet)

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Method: QL-ELE-00-002 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of Neomycin phosphotransferase II gene (ISO/FDIS 21569:2005).
Target:nptII

View other 59426 matches for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1930 2138 + 209 bp
Amplicon: 209 bp 
1     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
51    cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
101   acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
151   aggatgatca agagcatcag gggctcgcgc cagccgaact gttcgccagg
201   ctcaaggcg

View other 131 matches with an identical amplicon

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 21 bp 0 0 1 21 2.97e-09
primerB 100.0 21 bp 0 0 209 189 2.97e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.