Match details
Record ID:
LY667603.1
Database:
emblrelpat
Go to original record
Description:
KR 1020190003994-A/5: NUCLEIC ACID SEQUENCE FOR DETECTING EXISTENCE OF TRANSGENIC SOYBEAN EVENT DBN9004 IN BIOLOGICAL SAMPLE, KIT CONTAINING SAME AND DETECTION METHOD THEREFOR.
Taxon:
synthetic construct
Original size:
7059 bp
Patents:
KR1020190003994 (lens , espacenet )
View other 16 matches for this record
Method:
QT-CON-00-005
Go to GMOMETHODS record
View in JRC GMO-Matrix
Description: Quantitative PCR method for detection of the junction between the phosphinothricin N-acetyltransferase gene and the CaMV 35S terminator.
Target: pat-CaMV T-35S
View other 222 matches for this method
Predicted amplification details:
Mismatches
Gaps
From
To
Strand
Amplicon size
0
0
1438
1586
-
149 bp
1 gccagttagg ccagttaccc agatctgagt cgacctgcag gcatgcccgc
51 tgaaatcacc agtctctctc tacaaatcta tctctctcta taataatgtg
101 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctca
View other 150 matches with an identical amplicon
Primers and probe alignment:
Primer/Probe
% Identity
Length
Mismatches
Gaps
From
To
Score
primerA
100.0
21 bp
0
0
1
21
2.09e-09
primerB
100.0
24 bp
0
0
149
126
5.39e-11
probe
100.0
22 bp
0
0
36
57
6.20e-10
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.