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Food, Farming, Fisheries
European Union Reference Laboratory for Genetically Modified Food and Feed (EURL GMFF)

Match details

Record ID: LY667601.1
Database: emblrelpat Go to original record
Description: KR 1020190003994-A/3: NUCLEIC ACID SEQUENCE FOR DETECTING EXISTENCE OF TRANSGENIC SOYBEAN EVENT DBN9004 IN BIOLOGICAL SAMPLE, KIT CONTAINING SAME AND DETECTION METHOD THEREFOR.
Taxon: synthetic construct
Original size: 1207 bp
Patents: KR1020190003994 (lens, espacenet)

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Method: QT-EVE-GM-019 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Quantitative PCR method for detection of soybean event DBN-09004-6 (EURL GMFF, 2023).
Target:DBN-09004-6

View other 1 match for this method

Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 700 788 - 89 bp
Amplicon: 89 bp 
1     gccgtatccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
51    actctgtgac cccgtaatga aagcacacgt ttatggagc

View other 1 match with an identical amplicon

Primers and probe alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 4.42e-09
primerB 100.0 22 bp 0 0 89 68 3.87e-10
probe 100.0 21 bp 0 0 42 62 1.31e-09

Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.